Does this sound normal?

[grantx]

Here are a few more pictures.
5mm pitch lead screw
34 Nema
Drivers showing jumper positions (Off - On - On -Off)

[JAZZCNC]

Here are a few more pictures.
5mm pitch lead screw

Measuring like that can be misleading because the screw could be a twin start so that would be 2 x 5mm starts making it a 10mm pitch. This is why I said rotate and measure the distance it moved as that is the more accurate method.

[grantx]

You are right. I dont know what I measured before....
1 revolution gives 15mm travel on the Y axis (I havent measure the others)

When I tell it to move 100mm (G0 y100) it moves 150mm.

EDIT: Ok I remember how I measured it before. Using the auto-calibrate in Mach3 I get it to move 100 on the y-axis and it moves exactly that. It tells me the steps are 125, So that must be correct.

I dont know what that G0 y100 command is doing ...

[JAZZCNC]

You are right. I dont know what I measured before....
1 revolution gives 15mm travel on the Y axis (I havent measure the others)

When I tell it to move 100mm (G0 y100) it moves 150mm.

EDIT: Ok I remember how I measured it before. Using the auto-calibrate in Mach3 I get it to move 100 on the y-axis and it moves exactly that. It tells me the steps are 125, So that must be correct.

I dont know what that G0 y100 command is doing ...

Ok well, 15mm isn't a common pitch size but they do exist. However still, things don't add up.?

The drives are set for 2000 micro-steps and your direct drive to the motors. 2000/15=133.333333 Steps Per mm.

If we work it backward just in case the drives dip switches are telling porky's then from the Mach3 Auto calibrate setting 125 steps per with 15mm pitch this would give a micro-step setting of 1875.? I've never seen drives that allow this setting and the manual doesn't show it either.?

So something isn't adding up here at all.

Regards the G0 y100 command then it will move to y100 coordinate position which doesn't mean it will necessarily move 100mm. If you Zero the DRO then it should move 100mm.
To move a set amount without worrying about the coordinate location then you need to switch to incremental mode, try this next time.
G91 G0 y100 and it will move the amount asked for from the current location. When finished type G90 to put back into absolute positioning mode.

[JAZZCNC]

Drivers showing jumper positions (Off - On - On -Off)

Ok with those settings using the manual I'm looking at the micro-steps are set to 2000. This doesn't ring true to the mach3 steps per setting because it should be 400 if 5mm pitch and 200 if 10mm pitch. You have mach3 set at 125 Steps per which means you can not possibly be moving the correct distance unless the screw pitch is different to 5 or 10mm which I very much doubt.
The motors are 1.8degree so 200 steps per 360degree's and clearly direct drive so there are no ratios applied so that only leaves the screw pitch which can affect the movement and I'd be very very surprised if it's anything but 5mm or 10mm. This means it can't possibly be moving the correct distance unless the dip switch settings are wrong.